A Dumbbell-like Complex Formation for DNA Target Assay
Identifieur interne : 000235 ( France/Analysis ); précédent : 000234; suivant : 000236A Dumbbell-like Complex Formation for DNA Target Assay
Auteurs : Agnés Perrin [France] ; Thibault Martin [France] ; Alain Theretz [France]Source :
- Bioconjugate Chemistry [ 1043-1802 ] ; 2001.
Abstract
A simple and highly sensitive test for the detection of nucleic acid targets is described. It is based upon complex formation between a small-diameter magnetic particle and a larger and nonmagnetic particle through a hybridization reaction, what we have called a dumbbell-like complex. During the different steps, nonreacting nonmagnetic conjugates were eliminated by magnetic separation. At the end of the process, dumbbell complex number was estimated by counting under a microscope. Compared to the already described two-particle tests, our model was able to reach higher sensitivities, with a threshold typically in the amol/mL range (106 copies of HIV DNA/mL) without the need for complex instrumentation or genomic amplification reactions.
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DOI: 10.1021/bc000115s
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It is based upon complex formation between a small-diameter magnetic particle and a larger and nonmagnetic particle through a hybridization reaction, what we have called a dumbbell-like complex. During the different steps, nonreacting nonmagnetic conjugates were eliminated by magnetic separation. At the end of the process, dumbbell complex number was estimated by counting under a microscope. Compared to the already described two-particle tests, our model was able to reach higher sensitivities, with a threshold typically in the amol/mL range (106 copies of HIV DNA/mL) without the need for complex instrumentation or genomic amplification reactions.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Perrin, Agnes" sort="Perrin, Agnes" uniqKey="Perrin A" first="Agnés" last="Perrin">Agnés Perrin</name></region><name sortKey="Martin, Thibault" sort="Martin, Thibault" uniqKey="Martin T" first="Thibault" last="Martin">Thibault Martin</name><name sortKey="Theretz, Alain" sort="Theretz, Alain" uniqKey="Theretz A" first="Alain" last="Theretz">Alain Theretz</name><name sortKey="Theretz, Alain" sort="Theretz, Alain" uniqKey="Theretz A" first="Alain" last="Theretz">Alain Theretz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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